Stability of Mycophenolic Acid and Mycophenolic Acid Glucuronide in Human Plasma
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چکیده
منابع مشابه
IMP-dehydrogenase inhibition in human lymphocytes and lymphoblasts by mycophenolic acid and mycophenolic acid glucuronide.
Inosine 5'-monophosphate dehydrogenase (IMP-DH) activities were measured in human lymphocytes (exhibiting type I IMP-DH activity) and human lymphoblasts (exhibiting type II IMP-DH activity) in the presence of various amounts of mycophenolic acid (MPA) (0-20 mumol/L) and MPA glucuronide (MPAG) (0-200 mumol/L). Moreover, the influences of human serum albumin (HSA) and human plasma on the MPA- and...
متن کاملDetermination of the acyl glucuronide metabolite of mycophenolic acid in human plasma by HPLC and Emit.
BACKGROUND The acyl glucuronide (AcMPAG) of mycophenolic acid (MPA) has been found to possess pharmacologic and potentially proinflammatory activity in vitro. To establish its pharmacologic and toxicologic relevance in vivo, a reversed-phase HPLC method was modified to simultaneously determine MPA, the phenolic MPA-glucuronide (7-O-MPAG), and AcMPAG. In addition, cross-reactivity of AcMPAG in t...
متن کاملMycophenolic acid in silage.
We examined 233 silage samples and found that molds were present in 206 samples with counts between 1 x 10(3) and 8.9 x 10(7) (mean, 4.7 x 10(6)) CFU/g. Mycophenolic acid, a metabolite of Penicillium roqueforti, was detected by liquid chromatography-mass spectrometry in 74 (32%) of these samples at levels ranging from 20 to 35,000 (mean, 1,400) microg/kg. This compound has well-known immunosupp...
متن کاملQuantification of mycophenolic acid and glucuronide metabolite in human serum by HPLC-tandem mass spectrometry.
BACKGROUND The potent immunosuppressant mycophenolic acid (MPA) is metabolized to an inactive glucuronide (MPAG). The extent of metabolism varies among individuals, and the MPAG formed can be hydrolyzed to MPA and can displace MPA from serum albumin, creating a potential need to monitor both MPA and MPAG. METHODS After addition of the carboxybutoxy ether of MPA (MPAC) as internal standard, MP...
متن کاملAntitumor activity of mycophenolic acid.
n our laboratory in 4CS mice. (3) Mouse ascites hepatoma, MH134, was converted into ascitic form by Sato et al.*\ from a C3H/HeN mouse hapatoma induced after treatment with carbon tetrachloride by Andervont et al.5) It has been maintained in C3H/He mice with distinct strain specificity in tumor take in our laboratory. (4) Mouse ascites sarcoma, SR-C3H/He Th62, was originally induced with Schmid...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1999
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/45.1.127